2014yearNicoyalifestayEstablished in Canada, it is a technology company that is a key focus of the University of Waterloo's technology incubator and has received innovation funding support from the Canadian government. The company is committed to the research of sensor technology based on nanophotonics, and is committed to buildingProfessional tools that can assist scientists in their next major discovery2015Launch innovation at the end of the yearLSPRPatent technology (US patent number:US8693003B2）SystemOpenSPRThe molecular interactor provides a highly sensitive in vitro system for detecting the interactions between large and small molecules in biomedical basic research, drug development, tumor research, antibody development, and other fields.

OpenSPRofLSPRThe technology uses nano gold particles as the detection matrix, and through detectionLSPRResonance absorption peak shift, also known as wavelength change, is used to measure the interactions between molecules. The instrument is easy to operate, efficient, and accurate in detection,,LSPRTechnology differs from traditional methodsSPRBased on refractive indexSPRThe change in angle,LSPRTesting technology has many advantages:

l Not affected by changes in temperature and refractive index of buffer solution, and can be ignored“bulk”Effect, no need for dedicated reference channels, more stable and sensitive detection.

lEasy to operate,1Mastery within hours

lHigh performance: greater than90DaThe above molecules can be detected,

lEasy to maintain: The light source isLEDThe lamp and light path system are relatively simple, stable, and have a long lifespan

lThe sample ring is washable and replaceable, facilitating flexible application for different experimental requirements.

Localized surface plasmon resonance(LSPR)：

When the frequency of incident photons matches the overall vibration frequency of electrons conducted by noble metal nanoparticles, the nanoparticles will have a strong absorption effect on photon energy, resulting in localized surface plasmon resonance phenomenon（LSPR）When molecules in a solution bind to a fixed probe, it causes a change in the thickness of the biomolecule layer, resulting inLSPRThe absorption peak shifts, based onLSPRThe detection method can dynamically detect such real-time changes.

Nano gold particle technology

OpenSPRSensor chip

Covalent coupling sensor

Ordinary gold-Build your own surface chemistry

carboxyl-Used to couple many amino acid groups to ligands and fix any bandEDC NHSCoupled proteins

amino acid- Used for coupling activated carboxyl groups to ligands

Capture coupling sensor

Chain enzyme affinity- Used for coupling biotinylated ligands

Biotin- Ligand for coupling affinity tags of chain enzymes

Nitrotriacetic acid（NTA- Used for couplingHisLabel ligand)